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tampereen yliopisto: lääketieteen ja biotieteiden tiedekunta: tutkimus: vesa hytönen - proteiinidynamiikka:
Lääketieteen ja biotieteiden tiedekuntaTampereen yliopistoLääketieteen ja biotieteiden tiedekunta
Vesa Hytönen - Proteiinidynamiikka

Aiemmat julkaisut

Laitinen OH, Svedin E, Kapell S, Nurminen A, Hytönen VP, Flodström-Tullberg M
Enteroviral proteases: structure, host interactions and pathogenicity.
Rev Med Virol ;26(4)251-67, 2016
PMID

von Essen M, Rahikainen R, Oksala N, Raitoharju E, Seppälä I, Mennander A, Sioris T, Kholová I, Klopp N, Illig T, Karhunen PJ, Kähönen M, Lehtimäki T, Hytönen VP
Talin and vinculin are downregulated in atherosclerotic plaque; Tampere Vascular Study.
Atherosclerosis ;2016
PMID

Ciesielski GL, Hytönen VP, Kaguni LS
Biolayer Interferometry: A Novel Method to Elucidate Protein-Protein and Protein-DNA Interactions in the Mitochondrial DNA Replisome.
Methods Mol Biol ;2016
PMID

Tapio K, Leppiniemi J, Shen B, Hytönen VP, Fritzsche W, Toppari JJ
Toward Single Electron Nanoelectronics Using Self-Assembled DNA Structure.
Nano Lett ;16(11)6780-6786, 2016
PMID

Qi L, Jafari N, Li X, Chen Z, Li L, Hytönen VP, Goult BT, Zhan CG, Huang C
Talin2-mediated traction force drives matrix degradation and cell invasion.
J Cell Sci ;129(19)3661-3674, 2016
PMID

Qiu L, Wang W, Zhang A, Zhang N, Lemma T, Ge H, Toppari JJ, Hytönen VP, Wang J
Core-Shell Nanorod Columnar Array Combined with Gold Nanoplate-Nanosphere Assemblies Enable Powerful In Situ SERS Detection of Bacteria.
ACS Appl Mater Interfaces ;8(37)24394-403, 2016
PMID

Hynninen V, Vuori L, Hannula M, Tapio K, Lahtonen K, Isoniemi T, Lehtonen E, Hirsimäki M, Toppari JJ, Valden M, Hytönen VP
Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology.
Sci Rep ;2016
PMID

Haining AW, von Essen M, Attwood SJ, Hytönen VP, Del Río Hernández A
All Subdomains of the Talin Rod Are Mechanically Vulnerable and May Contribute To Cellular Mechanosensing.
ACS Nano ;10(7)6648-58, 2016
PMID

Hytönen VP, Wehrle-Haller B
Mechanosensing in cell-matrix adhesions - Converting tension into chemical signals.
Exp Cell Res ;343(1)35-41, 2016
PMID

Zolfaghari Emameh R, Barker HR, Tolvanen ME, Parkkila S, Hytönen VP
Horizontal transfer of β-carbonic anhydrase genes from prokaryotes to protozoans, insects, and nematodes.
Parasit Vectors ;2016
PMID

Lehtonen SI, Tullila A, Agrawal N, Kukkurainen S, Kähkönen N, Koskinen M, Nevanen TK, Johnson MS, Airenne TT, Kulomaa MS, Riihimäki TA, Hytönen VP
Artificial Avidin-Based Receptors for a Panel of Small Molecules.
ACS Chem Biol ;11(1)211-21, 2016
PMID

Orłowski A, Kukkurainen S, Pöyry A, Rissanen S, Vattulainen I, Hytönen VP, Róg T
PIP2 and Talin Join Forces to Activate Integrin.
J Phys Chem B 2015 ;119(38)12381-9
PMID

Oksala N, Pärssinen J, Seppälä I, Klopp N, Illig T, Laaksonen R, Levula M, Raitoharju E, Kholova I, Sioris T, Kähönen M, Lehtimäki T, Hytönen VP
Kindlin 3 (FERMT3) is associated with unstable atherosclerotic plaques, anti-inflammatory type II macrophages and upregulation of beta-2 integrins in all major arterial beds.
Atherosclerosis 2015 ;242(1)145-54
PMID

Koho T, Ihalainen TO, Stark M, Uusi-Kerttula H, Wieneke R, Rahikainen R, Blazevic V, Marjomäki V, Tampé R, Kulomaa MS, Hytönen VP
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Eur J Pharm Biopharm 2015 ;9622-31
PMID

Ray S, Steven RT, Green FM, Höök F, Taskinen B, Hytönen VP, Shard AG
Correction to "Neutralized Chimeric Avidin Binding at a Reference Biosensor Surface".
Langmuir 2015 ;31(22)6265
PMID

Pawlowski A, Moilanen AM, Rissanen IA, Määttä JA, Hytönen VP, Ihalainen JA, Bamford JK
The Minor Capsid Protein VP11 of Thermophilic Bacteriophage P23-77 Facilitates Virus Assembly by Using Lipid-Protein Interactions.
J Virol 2015 ;89(15)7593-603
PMID

Ray S, Steven RT, Green FM, Höök F, Taskinen B, Hytönen VP, Shard AG
Neutralized chimeric avidin binding at a reference biosensor surface.
Langmuir 2015 ;31(6)1921-30
PMID

Lehtonen SI, Taskinen B, Ojala E, Kukkurainen S, Rahikainen R, Riihimäki TA, Laitinen OH, Kulomaa MS, Hytönen VP
Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning.
Protein Eng Des Sel 2015 ;28(1)23-8
PMID

Ribeiro C, Pärssinen J, Sencadas V, Correia V, Miettinen S, Hytönen VP, Lanceros-Méndez S
Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells.
J Biomed Mater Res A 2015 ;103(6)2172-5
PMID

Pärssinen J, Hammarén H, Rahikainen R, Sencadas V, Ribeiro C, Vanhatupa S, Miettinen S, Lanceros-Méndez S, Hytönen VP
Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride).
J Biomed Mater Res A 2015 ;103(3)919-28
PMID

Taskinen B, Zauner D, Lehtonen SI, Koskinen M, Thomson C, Kähkönen N, Kukkurainen S, Määttä JA, Ihalainen TO, Kulomaa MS, Gruber HJ, Hytönen VP
Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity.
Bioconjug Chem 2014 ;25(12)2233-43
PMID

Ribeiro C, Pärssinen J, Sencadas V, Correia V, Miettinen S, Hytönen VP, Lanceros-Méndez S
Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells.
J Biomed Mater Res A 2014 ;
PMID

Takala H, Lehtivuori H, Hammarén H, Hytönen VP, Ihalainen JA
Connection between Absorption Properties and Conformational Changes in Deinococcus radiodurans Phytochrome.
Biochemistry 2014 ;53(45)7076-85
PMID

Vuori L, Leppiniemi J, Hannula M, Lahtonen K, Hirsimäki M, Nõmmiste E, Costelle L, Hytönen VP, Valden M
Biofunctional hybrid materials: bimolecular organosilane monolayers on FeCr alloys.
Nanotechnology 2014 ;25(43)435603
PMID

Kukkurainen S, Määttä JA, Saeger J, Valjakka J, Vogel V, Hytönen VP
The talin-integrin interface under mechanical stress.
Mol Biosyst 2014 ;10(12)3217-28
PMID

Zolfaghari Emameh R, Barker H, Hytönen VP, Tolvanen ME, Parkkila S
Beta carbonic anhydrases: novel targets for pesticides and anti-parasitic agents in agriculture and livestock husbandry.
Parasit Vectors 2014 ;7403
PMID

Rosilo H, McKee JR, Kontturi E, Koho T, Hytönen VP, Ikkala O, Kostiainen MA
Cationic polymer brush-modified cellulose nanocrystals for high-affinity virus binding.
Nanoscale 2014 ;6(20)11871-81
PMID

Deng Y, Alicea-Velázquez NL, Bannwarth L, Lehtonen SI, Boggon TJ, Cheng HC, Hytönen VP, Turk BE
Global analysis of human nonreceptor tyrosine kinase specificity using high-density Peptide microarrays.
J Proteome Res 2014 ;13(10)4339-46
PMID

Köhler M, Karner A, Leitner M, Hytönen VP, Kulomaa M, Hinterdorfer P, Ebner A
pH-dependent deformations of the energy landscape of avidin-like proteins investigated by single molecule force spectroscopy.
Molecules 2014 ;19(8)12531-46
PMID

Tossavainen H, Kukkurainen S, Määttä JA, Kähkönen N, Pihlajamaa T, Hytönen VP, Kulomaa MS, Permi P
Chimeric Avidin--NMR structure and dynamics of a 56 kDa homotetrameric thermostable protein.
PLoS One 2014 ;9(6)e100564
PMID

Kurppa K, Hytönen VP, Nakari-Setälä T, Kulomaa MS, Linder MB
Molecular engineering of avidin and hydrophobin for functional self-assembling interfaces.
Colloids Surf B Biointerfaces 2014 ;120102-9
PMID

Pärssinen J, Hammarén H, Rahikainen R, Sencadas V, Ribeiro C, Vanhatupa S, Miettinen S, Lanceros-Méndez S, Hytönen VP
Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride).
J Biomed Mater Res A 2014 ;
PMID

Pinon P, Pärssinen J, Vazquez P, Bachmann M, Rahikainen R, Jacquier MC, Azizi L, Määttä JA, Bastmeyer M, Hytönen VP, Wehrle-Haller B
Talin-bound NPLY motif recruits integrin-signaling adapters to regulate cell spreading and mechanosensing.
J Cell Biol 2014 ;205(2)265-81
PMID

Taskinen B, Airenne TT, Jänis J, Rahikainen R, Johnson MS, Kulomaa MS, Hytönen VP
A novel chimeric avidin with increased thermal stability using DNA shuffling.
PLoS One 2014 ;9(3)e92058
PMID

Koho T, Koivunen MR, Oikarinen S, Kummola L, Mäkinen S, Mähönen AJ, Sioofy-Khojine A, Marjomäki V, Kazmertsuk A, Junttila I, Kulomaa MS, Hyöty H, Hytönen VP, Laitinen OH
Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice.
Antiviral Res 2014 ;10493-101
PMID

Hytönen VP, Wehrle-Haller B
Protein conformation as a regulator of cell-matrix adhesion.
Phys Chem Chem Phys 2014 ;16(14)6342-57
PMID

Rangl M, Leitner M, Riihimäki T, Lehtonen S, Hytönen VP, Gruber HJ, Kulomaa M, Hinterdorfer P, Ebner A
Investigating the binding behaviour of two avidin-based testosterone binders using molecular recognition force spectroscopy.
J Mol Recognit 2014 ;27(2)92-7
PMID

Näreoja T, Ebner A, Gruber HJ, Taskinen B, Kienberger F, Hänninen PE, Hytönen VP, Hinterdorfer P, Härmä H
Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay.
Anal Bioanal Chem 2014 ;406(2)493-503
PMID

Näreoja T, Ebner A, Gruber HJ, Taskinen B, Kienberger F, Hänninen PE, Hytönen VP, Hinterdorfer P, Härmä H
Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay.
Anal Bioanal Chem 2013 ;
PMID

Taskinen B, Zmurko J, Ojanen M, Kukkurainen S, Parthiban M, Määttä JA, Leppiniemi J, Jänis J, Parikka M, Turpeinen H, Rämet M, Pesu M, Johnson MS, Kulomaa MS, Airenne TT, Hytönen VP
Zebavidin - an avidin-like protein from zebrafish.
PLoS One 2013 ;8(10)e77207
PMID

Turpeinen H, Oksanen A, Kivinen V, Kukkurainen S, Uusimäki A, Rämet M, Parikka M, Hytönen VP, Nykter M, Pesu M
Proprotein Convertase Subtilisin/Kexin Type 7 (PCSK7) Is Essential for the Zebrafish Development and Bioavailability of Transforming Growth Factor β1a (TGFβ1a).
J Biol Chem 2013 ;288(51)36610-23
PMID

Pollheimer P, Taskinen B, Scherfler A, Gusenkov S, Creus M, Wiesauer P, Zauner D, Schöfberger W, Schwarzinger C, Ebner A, Tampé R, Stutz H, Hytönen VP, Gruber HJ
Reversible biofunctionalization of surfaces with a switchable mutant of avidin.
Bioconjug Chem 2013 ;24(10)1656-68
PMID

Leppiniemi J, Meir A, Kähkönen N, Kukkurainen S, Määttä JA, Ojanen M, Jänis J, Kulomaa MS, Livnah O, Hytönen VP
The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins.
Protein Sci 2013 ;22(7)980-94
PMID

Tossavainen H, Helppolainen SH, Määttä JA, Pihlajamaa T, Hytönen VP, Kulomaa MS, Permi P
Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms.
Biomol NMR Assign 2013 ;7(1)35-8
PMID

Saeger J, Hytönen VP, Klotzsch E, Vogel V
GFP's mechanical intermediate states.
PLoS One 2012 ;7(10)e46962
PMID

Albers WM, Pelto JM, Suspène C, Määttä JA, Yassar A, Hytönen VP, Vikholm-Lundin IM, Tappura K
Structural and functional characteristics of chimeric avidins physically adsorbed onto functionalized polythiophene thin films.
ACS Appl Mater Interfaces 2012 ;4(8)4067-77
PMID

Durdagi S, Vullo D, Pan P, Kähkönen N, Määttä JA, Hytönen VP, Scozzafava A, Parkkila S, Supuran CT
Protein-protein interactions: inhibition of mammalian carbonic anhydrases I-XV by the murine inhibitor of carbonic anhydrase and other members of the transferrin family.
J Med Chem 2012 ;55(11)5529-35
PMID

Leppiniemi J, Grönroos T, Määttä JA, Johnson MS, Kulomaa MS, Hytönen VP, Airenne TT
Structure of bradavidin-C-terminal residues act as intrinsic ligands.
PLoS One 2012 ;7(5)e35962
PMID

Koho T, Mäntylä T, Laurinmäki P, Huhti L, Butcher SJ, Vesikari T, Kulomaa MS, Hytönen VP
Purification of norovirus-like particles (VLPs) by ion exchange chromatography.
J Virol Methods 2012 ;181(1)6-11
PMID

Niederhauser B, Siivonen J, Määttä JA, Jänis J, Kulomaa MS, Hytönen VP
DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools.
J Biotechnol 2012 ;157(1)38-49
PMID

Tamminen K, Huhti L, Koho T, Lappalainen S, Hytönen VP, Vesikari T, Blazevic V
A comparison of immunogenicity of norovirus GII-4 virus-like particles and P-particles.
Immunology 2012 ;135(1)89-99
PMID

Koho T, Huhti L, Blazevic V, Nurminen K, Butcher SJ, Laurinmäki P, Kalkkinen N, Rönnholm G, Vesikari T, Hytönen VP, Kulomaa MS
Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus.
J Virol Methods 2012 ;179(1)1-7
PMID

Linko V, Leppiniemi J, Shen B, Niskanen E, Hytönen VP, Toppari JJ
Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules.
Nanoscale 2011 ;3(9)3788-92
PMID

Heikkinen JJ, Kivimäki L, Määttä JA, Mäkelä I, Hakalahti L, Takkinen K, Kulomaa MS, Hytönen VP, Hormi OE
Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates.
Colloids Surf B Biointerfaces 2011 ;87(2)409-14
PMID

Bootorabi F, Jänis J, Hytönen VP, Valjakka J, Kuuslahti M, Vullo D, Niemelä O, Supuran CT, Parkkila S
Acetaldehyde-derived modifications on cytosolic human carbonic anhydrases.
J Enzyme Inhib Med Chem 2011 ;26(6)862-70
PMID

Riihimäki TA, Hiltunen S, Rangl M, Nordlund HR, Määttä JA, Ebner A, Hinterdorfer P, Kulomaa MS, Takkinen K, Hytönen VP
Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein.
BMC Biotechnol 2011 ;1164
PMID

Riihimäki TA, Kukkurainen S, Varjonen S, Hörhä J, Nyholm TK, Kulomaa MS, Hytönen VP
Construction of chimeric dual-chain avidin by tandem fusion of the related avidins.
PLoS One 2011 ;6(5)e20535
PMID

Linko V, Leppiniemi J, Paasonen ST, Hytönen VP, Toppari JJ
Defined-size DNA triple crossover construct for molecular electronics: modification, positioning and conductance properties.
Nanotechnology 2011 ;22(27)275610
PMID

Heikkinen JJ, Riihimäki TA, Määttä JA, Suomela SE, Kantomaa J, Kulomaa MS, Hytönen VP, Hormi OE
Covalent biofunctionalization of cellulose acetate with thermostable chimeric avidin.
ACS Appl Mater Interfaces 2011 ;3(7)2240-5
PMID

Leppiniemi J, Määttä JA, Hammaren H, Soikkeli M, Laitaoja M, Jänis J, Kulomaa MS, Hytönen VP
Bifunctional avidin with covalently modifiable ligand binding site.
PLoS One 2011 ;6(1)e16576
PMID

Nurminen K, Blazevic V, Huhti L, Räsänen S, Koho T, Hytönen VP, Vesikari T
Prevalence of norovirus GII-4 antibodies in Finnish children.
J Med Virol 2011 ;83(3)525-31
PMID

Määttä JA, Eisenberg-Domovich Y, Nordlund HR, Hayouka R, Kulomaa MS, Livnah O, Hytönen VP
Chimeric avidin shows stability against harsh chemical conditions--biochemical analysis and 3D structure.
Biotechnol Bioeng 2011 ;108(3)481-90
PMID

Huhti L, Blazevic V, Nurminen K, Koho T, Hytönen VP, Vesikari T
A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles.
Arch Virol 2010 ;155(11)1855-8
PMID

Syrjänen L, Tolvanen M, Hilvo M, Olatubosun A, Innocenti A, Scozzafava A, Leppiniemi J, Niederhauser B, Hytönen VP, Gorr TA, Parkkila S, Supuran CT
Characterization of the first beta-class carbonic anhydrase from an arthropod (Drosophila melanogaster) and phylogenetic analysis of beta-class carbonic anhydrases in invertebrates.
BMC Biochem 2010 ;1128
PMID

Saltel F, Mortier E, Hytönen VP, Jacquier MC, Zimmermann P, Vogel V, Liu W, Wehrle-Haller B
New PI(4,5)P2- and membrane proximal integrin-binding motifs in the talin head control beta3-integrin clustering.
J Cell Biol 2009 ;187(5)715-31
PMID

Määttä JA, Helppolainen SH, Hytönen VP, Johnson MS, Kulomaa MS, Airenne TT, Nordlund HR
Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalis.
BMC Struct Biol 2009 ;963
PMID

Meir A, Helppolainen SH, Podoly E, Nordlund HR, Hytönen VP, Määttä JA, Wilchek M, Bayer EA, Kulomaa MS, Livnah O
Crystal structure of rhizavidin: insights into the enigmatic high-affinity interaction of an innate biotin-binding protein dimer.
J Mol Biol 2009 ;386(2)379-90
PMID

Määttä JA, Airenne TT, Nordlund HR, Jänis J, Paldanius TA, Vainiotalo P, Johnson MS, Kulomaa MS, Hytönen VP
Rational modification of ligand-binding preference of avidin by circular permutation and mutagenesis.
Chembiochem 2008 ;9(7)1124-35
PMID

Hayouka R, Eisenberg-Domovich Y, Hytönen VP, Määttä JA, Nordlund HR, Kulomaa MS, Wilchek M, Bayer EA, Livnah O
Critical importance of loop conformation to avidin-enhanced hydrolysis of an active biotin ester.
Acta Crystallogr D Biol Crystallogr 2008 ;64(Pt 3)302-8
PMID

Hytönen VP, Vogel V
How force might activate talin's vinculin binding sites: SMD reveals a structural mechanism.
PLoS Comput Biol 2008 ;4(2)e24
PMID

Laitinen OH, Nordlund HR, Hytönen VP, Kulomaa MS
Brave new (strept)avidins in biotechnology.
Trends Biotechnol 2007 ;25(6)269-77
PMID

Hytönen VP, Määttä JA, Niskanen EA, Huuskonen J, Helttunen KJ, Halling KK, Nordlund HR, Rissanen K, Johnson MS, Salminen TA, Kulomaa MS, Laitinen OH, Airenne TT
Structure and characterization of a novel chicken biotin-binding protein A (BBP-A).
BMC Struct Biol 2007 ;78
PMID

Rinne J, Albarran B, Jylhävä J, Ihalainen TO, Kankaanpää P, Hytönen VP, Stayton PS, Kulomaa MS, Vihinen-Ranta M
Internalization of novel non-viral vector TAT-streptavidin into human cells.
BMC Biotechnol 2007 ;71
PMID

Laitinen OH, Hytönen VP, Nordlund HR, Kulomaa MS
Genetically engineered avidins and streptavidins.
Cell Mol Life Sci 2006 ;63(24)2992-3017
PMID

Repo S, Paldanius TA, Hytönen VP, Nyholm TK, Halling KK, Huuskonen J, Pentikäinen OT, Rissanen K, Slotte JP, Airenne TT, Salminen TA, Kulomaa MS, Johnson MS
Binding properties of HABA-type azo derivatives to avidin and avidin-related protein 4.
Chem Biol 2006 ;13(10)1029-39
PMID

Tuukkanen S, Toppari JJ, Kuzyk A, Hirviniemi L, Hytönen VP, Ihalainen T, Törmä P
Carbon nanotubes as electrodes for dielectrophoresis of DNA.
Nano Lett 2006 ;6(7)1339-43
PMID

Hytönen VP, Hörhä J, Airenne TT, Niskanen EA, Helttunen KJ, Johnson MS, Salminen TA, Kulomaa MS, Nordlund HR
Controlling quaternary structure assembly: subunit interface engineering and crystal structure of dual chain avidin.
J Mol Biol 2006 ;359(5)1352-63
PMID

Prizant M, Eisenberg-Domovich Y, Hytönen VP, Kulomaa MS, Wilchek M, Bayer EA, Livnah O
Factors dictating the pseudocatalytic efficiency of avidins.
J Mol Biol 2006 ;358(3)754-63
PMID

Hytönen VP, Määttä JA, Kidron H, Halling KK, Hörhä J, Kulomaa T, Nyholm TK, Johnson MS, Salminen TA, Kulomaa MS, Airenne TT
Avidin related protein 2 shows unique structural and functional features among the avidin protein family.
BMC Biotechnol 2005 ;528
PMID

Hytönen VP, Nordlund HR, Hörhä J, Nyholm TK, Hyre DE, Kulomaa T, Porkka EJ, Marttila AT, Stayton PS, Laitinen OH, Kulomaa MS
Dual-affinity avidin molecules.
Proteins 2005 ;61(3)597-607
PMID

Nordlund HR, Hytönen VP, Hörhä J, Määttä JA, White DJ, Halling K, Porkka EJ, Slotte JP, Laitinen OH, Kulomaa MS
Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins.
Biochem J 2005 ;392(Pt 3)485-91
PMID

Eisenberg-Domovich Y, Hytönen VP, Wilchek M, Bayer EA, Kulomaa MS, Livnah O
High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability.
Acta Crystallogr D Biol Crystallogr 2005 ;61(Pt 5)528-38
PMID

Niskanen EA, Hytönen VP, Grapputo A, Nordlund HR, Kulomaa MS, Laitinen OH
Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family.
BMC Genomics 2005 ;6(1)41
PMID

Laitinen OH, Airenne KJ, Hytönen VP, Peltomaa E, Mähönen AJ, Wirth T, Lind MM, Mäkelä KA, Toivanen PI, Schenkwein D, Heikura T, Nordlund HR, Kulomaa MS, Ylä-Herttuala S
A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo.
Nucleic Acids Res 2005 ;33(4)e42
PMID

Nordlund HR, Hytönen VP, Laitinen OH, Kulomaa MS
Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum.
J Biol Chem 2005 ;280(14)13250-5
PMID

Hytönen VP, Määttä JA, Nyholm TK, Livnah O, Eisenberg-Domovich Y, Hyre D, Nordlund HR, Hörhä J, Niskanen EA, Paldanius T, Kulomaa T, Porkka EJ, Stayton PS, Laitinen OH, Kulomaa MS
Design and construction of highly stable, protease-resistant chimeric avidins.
J Biol Chem 2005 ;280(11)10228-33
PMID

Hytönen VP, Laitinen OH, Airenne TT, Kidron H, Meltola NJ, Porkka EJ, Hörhä J, Paldanius T, Määttä JA, Nordlund HR, Johnson MS, Salminen TA, Airenne KJ, Ylä-Herttuala S, Kulomaa MS
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli.
Biochem J 2004 ;384(Pt 2)385-90
PMID

Nordlund HR, Laitinen OH, Hytönen VP, Uotila ST, Porkka E, Kulomaa MS
Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins.
J Biol Chem 2004 ;279(35)36715-9
PMID

Räty JK, Airenne KJ, Marttila AT, Marjomäki V, Hytönen VP, Lehtolainen P, Laitinen OH, Mähönen AJ, Kulomaa MS, Ylä-Herttuala S
Enhanced gene delivery by avidin-displaying baculovirus.
Mol Ther 2004 ;9(2)282-91
PMID

Hytönen VP, Nyholm TK, Pentikäinen OT, Vaarno J, Porkka EJ, Nordlund HR, Johnson MS, Slotte JP, Laitinen OH, Kulomaa MS
Chicken avidin-related protein 4/5 shows superior thermal stability when compared with avidin while retaining high affinity to biotin.
J Biol Chem 2004 ;279(10)9337-43
PMID

Nordlund HR, Hytönen VP, Laitinen OH, Uotila ST, Niskanen EA, Savolainen J, Porkka E, Kulomaa MS
Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding.
FEBS Lett 2003 ;555(3)449-54
PMID

Airenne KJ, Peltomaa E, Hytönen VP, Laitinen OH, Ylä-Herttuala S
Improved generation of recombinant baculovirus genomes in Escherichia coli.
Nucleic Acids Res 2003 ;31(17)e101
PMID

Hytönen VP, Laitinen OH, Grapputo A, Kettunen A, Savolainen J, Kalkkinen N, Marttila AT, Nordlund HR, Nyholm TK, Paganelli G, Kulomaa MS
Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment.
Biochem J 2003 ;372(Pt 1)219-25
PMID

Laitinen OH, Nordlund HR, Hytönen VP, Uotila ST, Marttila AT, Savolainen J, Airenne KJ, Livnah O, Bayer EA, Wilchek M, Kulomaa MS
Rational design of an active avidin monomer.
J Biol Chem 2003 ;278(6)4010-4
PMID

Nordlund HR, Laitinen OH, Uotila ST, Nyholm T, Hytönen VP, Slotte JP, Kulomaa MS
Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces.
J Biol Chem 2003 ;278(4)2479-83
PMID

Marttila AT, Hytönen VP, Laitinen OH, Bayer EA, Wilchek M, Kulomaa MS
Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences.
Biochem J 2003 ;369(Pt 2)249-54
PMID

Laitinen OH, Hytönen VP, Ahlroth MK, Pentikäinen OT, Gallagher C, Nordlund HR, Ovod V, Marttila AT, Porkka E, Heino S, Johnson MS, Airenne KJ, Kulomaa MS
Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.
Biochem J 2002 ;363(Pt 3)609-17
PMID

 
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